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Rabbit serum against K1 peptide, an immunogenic epitope of the Trypanosoma cruzi KMP-11, decreases parasite invasion to cells

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Autor Diaz-Soto J.C.
Autor Lasso P.
Autor Guzman F.
Autor Forero-Shelton M.
Autor Thomas M.D.C.
Autor Lopez M.C.
Autor Guhl F.
Autor Cuellar A.
Autor Puerta C.J.
Autor Gonzalez J.M.
Fecha Ingreso 2014-04-05T00:23:19Z
Fecha Disponible 2014-04-05T00:23:19Z
Fecha en Repositorio 2014-04-04
dc.identifier 10.1016/j.actatropica.2012.05.015
dc.description.abstract KMP-11 is a highly conserved protein of . Trypanosoma cruzi implicated in parasite's motility. Here we show that K1, a peptide derived from KMP-11, induced polyclonal antibodies capable of decreasing . T. cruzi infection in vitro. Rabbit sera rose against K1 peptide showed recognition of the recombinant protein by ELISA and Western blot and also of the native protein in both epimastigotes and trypomastigotes as evaluated by immunofluorescence test and flow cytometry. Invasion assays showed a significant reduction of trypomastigotes infection of eukaryotic cells when parasites were pre-incubated with anti-K1 rabbit serum. Computational modeling predicted that the K1 sequence conserved its α-helical configuration into the protein, and some of the amino acid residues appear accessible for recognition by antibodies in vivo. Taken together, these results support the idea that the K1 peptide induces antibodies than can have a potential role in protective immunity in Chagas disease. © 2012 Elsevier B.V. en_US
dc.source Acta Tropica
Link Descarga dc.source.uri
Title dc.title Rabbit serum against K1 peptide, an immunogenic epitope of the Trypanosoma cruzi KMP-11, decreases parasite invasion to cells en_US
Tipo dc.type Article
dc.description.keywords amino acid; epitope; kinetoplastid membrane protein 11; peptide antibody; polyclonal antibody; recombinant protein; structural protein; threonylleucylglutamylglutamylphenylalanylserylalanyllysylleucine; threonylleucylglutamylglutamylphenylalanylserylalanyllysylleucine antibody; unclassified drug; amino acid; antibody; computer simulation; disease control; parasite; peptide; protein; amino acid sequence; antibody detection; antibody production; antigen recognition; article; cell invasion; Chagas disease; controlled study; epimastigote; eukaryotic cell; immunity; in vitro study; in vivo study; mathematical model; nonhuman; parasite transmission; protein expression; protein function; protein induction; protein structure; protein synthesis; rabbit; Trypanosoma cruzi; trypomastigote; Animals; Antibodies, Protozoan; Antigens, Protozoan; Antiprotozoal Agents; Biological Agents; Cercopithecus aethiops; Epitopes; Parasitic Sensitivity Tests; Rabbits; Trypanosoma cruzi; Vero Cells; Eukaryota; Oryctolagus cuniculus; Trypanosoma cruzi en_US

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