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Effect of cholesterol content on the structural and dynamic membrane properties of DMPC/DSPC large unilamellar bilayers

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Autor Soto-Arriaza M.A.
Autor Olivares-Ortega C.
Autor Quina F.H.
Autor Aguilar L.F.
Autor Sotomayor C.P.
Fecha Ingreso 2014-04-05T00:22:20Z
Fecha Disponible 2014-04-05T00:22:20Z
Fecha en Repositorio 2014-04-04
dc.identifier 10.1016/j.bbamem.2013.07.031
dc.description.abstract In this study, we report the effect of cholesterol content on the dynamic and structural properties of a dimyristoyl-phosphatidylcholine and distearoyl-phosphatidylcholine mixture in large unilamellar vesicles. The range of cholesterol concentrations studied varied around approximately 33.3 mol%, where it has been postulated that an abrupt change in bilayer organization occurs. Steady-state fluorescence measurements demonstrated a typical behavior; at low temperatures in the main phase transition, the cholesterol concentration did not affect the gel phase, but at 37.5 C (phase coexistence) and in the liquid crystalline phase, the presence of cholesterol produced an increase in the fluorescence anisotropy of DPH and the generalized polarization of Laurdan. The greater effect was observed in the liquid crystalline phase, in which the bilayer became a mixture of fluid-like and liquid-ordered phases. The results obtained at approximately 33.3 mol% of Cholesterol demonstrated that the Generalized Polarization of Laurdan, the DPH lifetime, the limiting anisotropy and the rotational correlation time, as well as the fluorescence quenching of DPH by TEMPO, are at maxima, while the fluorescence intensity of dehydroergosterol and the lipid solubility in TritonX-100 are at minima. These results correlate well with the hypothesis of domain segregation in the DMPC/DSPC/Cholesterol LUV system. In this context, we postulate that at 33.3 mol% of Cho, the proportion of ordered domains reaches a maximum. © 2013 Elsevier B.V. en_US
dc.source Biochimica et Biophysica Acta - Biomembranes
Link Descarga dc.source.uri
Title dc.title Effect of cholesterol content on the structural and dynamic membrane properties of DMPC/DSPC large unilamellar bilayers en_US
Tipo dc.type Article
dc.description.keywords 1,6 diphenyl 1,3,5 hexatriene; 2,2,6,6 tetramethyl 1 piperidinyloxy; cholesterol; dehydroergosterol; dimyristoylphosphatidylcholine; distearoylphosphatidylcholine; ergosterol; piperidine derivative; unclassified drug; anisotropy; article; bilayer membrane; controlled study; fluorescence analysis; lipid analysis; lipid bilayer; lipid solubility; low temperature procedures; membrane model; membrane permeability; membrane structure; membrane vesicle; phase transition; photochemical quenching; priority journal; steady state; temperature sensitivity; 1,6-diphenyl-1,3,5-hexatriene; 2,2,6,6-tetramethyl-1-piperidinyloxy; 2-dimethylamino-6-lauroylnaphthalene; C(12)E(8); Cho; Cholesterol; cholesterol; dehydroergosterol; DHE; dimyristoyl-phosphatidylcholine; dioleoyl-phosphatidylcholine; distearoyl-phosphatidylcholine; DMPC; DOPC; DPH; DSPC; generalized polarization; GP; large unilamellar vesicles; Large unilamellar vesicles; Laurdan; Lipid domain; LUVs; octaethylene glycol monododecyl ether; Segregated domain; TEMPO; Cell Membrane; Cholesterol; Detergents; Dimyristoylphosphatidylcholine; Fluorescence Polarization; Lipid Bilayers; Phosphatidylcholines; Unilamellar Liposomes en_US

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