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ISPsa2, the first mobile genetic element to be described and characterized in the bacterial facultative intracellular pathogen Piscirickettsia salmonis

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Autor Marshall S.H.
Autor Henriquez V.
Autor Gomez F.A.
Autor Cardenas C.
Fecha Ingreso 2014-04-05T00:16:59Z
Fecha Disponible 2014-04-05T00:16:59Z
Fecha en Repositorio 2014-04-04
dc.identifier 10.1111/j.1574-6968.2010.02132.x
dc.description.abstract Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassified recently as a facultative intracellular Gammaproteobacteria. This designation has prompted the independent growth of the bacterium to a pure state for detailed study of its biology, genetics and epidemiology, properties that are still relatively poorly characterized. The preliminary sequence analysis of a 992-bp fragment of pure P. salmonis DNA allowed us to characterize a novel and complete 863-bp insertion sequence in the bacterial genome (named ISPsa2), which has a novel 16/16 bp perfectly inverted terminal repeat flanking a 726-bp ORF that encodes a putative transposase (Tnp-Psa). The coding sequence of the enzyme shares similarities to that described in some Bacillus species and particularly to those of the IS6 family. ISPsa2 carries its own promoter with standard -10 and -35 sequences, suggesting an interesting potential for plasticity in this pathogenic bacterium. Additionally, the presence of ISPsa2 was confirmed from three isolates of P. salmonis collected from different epizootics in Chile in 2010. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. en_US
dc.source FEMS Microbiology Letters
Link Descarga dc.source.uri
Title dc.title ISPsa2, the first mobile genetic element to be described and characterized in the bacterial facultative intracellular pathogen Piscirickettsia salmonis en_US
Tipo dc.type Letter
dc.description.keywords bacterial DNA; genomic DNA; protein ISPsa2; transposase; unclassified drug; Bacillus; bacterial genome; bacterial growth; bacterial metabolism; bacterium isolate; controlled study; DNA flanking region; DNA sequence; gene insertion sequence; genome size; inverted repeat; ISPsa2 gene; letter; nonhuman; nucleotide sequence; open reading frame; Piscirickettsia salmonis; priority journal; promoter region; sequence homology; zoonosis; Amino Acid Sequence; Animals; Bacterial Proteins; Base Sequence; DNA Transposable Elements; Fish Diseases; Molecular Sequence Data; Piscirickettsia; Piscirickettsiaceae Infections; Promoter Regions, Genetic; Salmon; Sequence Alignment; Transposases; Alphaproteobacteria; Bacillus (bacterium); Bacteria (microorganisms); Gammaproteobacteria; Negibacteria; Piscirickettsia salmonis; Rickettsia en_US

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